	   SnapGene      GGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGTATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATTGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCTAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAACATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAAGAATTAATTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGAAATTGTAAACGTTACTAGTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCAATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCAGTGGTGGTGGTGGTGGTGCTCGAGTGCGGCCGCAAGCTTGTCGACGGCGCTCGAATTCGGATCCGTTATCCACTTCCAATATTGGATTGGAAGTACAGGTTTTCCTCGATCCCACCAATCTGTTCTCTGTGAGCCTCAATAATATCGTTATCCTCCATGTCCAAATCTTCAGGGGTCTGATCAGCTTGAATTCTAATACCGTCGTACAAGAATCTTAAGGAGTCCATTTCCTTACCCTGTCTTTTAGCGAACGCTTCCATCAGCCTTCTTAAAGGAGTGGTCTTTTTGATCTTGAAGAAGATCTCTGAAGATCCATCGGACACCTTTAAATTGATGTGAGTCTCAGGCTTGACTTCTGGCTTGACCTCTGGCTTAGCTTCTTGATTGACTTCTGAGTCCGACATGCTAGCCATAGAAGAACCATGGTGATGGTGATGGTGAGAAGAACCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAA  27                  "         *            /            
               #         "                                                                         )         /               ,                                                             #                           +      "                                                                                                 	                                 $   )                
                           	         	                                                                  %   $                            %                                                                         #      (      !         !                  
                     ,               	                        #                                     	                           ì 8ùò¦	 
wo‘Ò£�ßwÂÃ ¡ ÚÈóðË	
Œ
:x¹ÓòàxÄ-ö 5 c9èò§	
P
p’¤ž©„œ¡ e � í "§sÜú8Z
k
PdTÙ��Í9÷‰¼  �Î¦ÉuºÌ!.�"  §ºÂãþ#©ñ&	·	
�
ë
;yœÁ$®ÔîóJáys÷ & ’ ¢ Ý Ûàî4ÁôÖ¯†	Ì	@
A°Hkê,E û Ms&.‹ú“@//Å@* � =R	€‰éóZz ÉßY	
t
dTÌ™¼ô÷úý š ¦P¯ô…ˆ¶Ó�¨«Ø 6 ›  ¨ÄÙiƒ¨¾	2	S	Q
¶
Kù¶ª_´~  M ”:F£á\	#Œâç„q¥SU dËœ=¬¯&¨	Ó	Ö
“!Ÿ˜¢/   _ f Rnàý5Ýïû‡½Ö<}œùÆÌQeÜåUG\¤ÎîñN6O�ÁÕ>ŠÑ î #‹¨ÔXø"tÑ=5›A	l
°
{ç¿>²d*:è ‚ ,1ø(·J9í[
ä
	èÚKWc‚�øàä v’ë ø0â*
Ýk›:¶¾þÜc½
 3 q$Ïx¼u—™Ý ïã§JvÑ
è©/8w  { .LxÒ-	ž
ð—Ï,+ÍC{"y­ ®ñ‚aŒ’)bÊ<À[¸

 D»Ét—1� ± åkÃªòÚ÷	�	Ž
RïôK$�«zt¼Ç'x# @E¡»¾äŒ_xÿ€'	~	¼	
©
<g $zã_…“áTÕê÷ý‘îø / ¨NR˜Jìì
U×Û�8Tÿ&­ÃâJ‹ e¹8$E¸	Ù	Â%:w¯ßê“®Ö¸" G \ æ‹ï5_ƒÂlA
•
Å
Î
Ó
sëú�")h>�£t¤	±K “ £ « Zõ•×b
c©ÿI
li
 Þ z2A°s	Í	0
G
„
^–ôBWÃFpK„ W ÜŽá<	‡	’	š	3µ±ë-]¤'"}:”ü ¨ ˆ/†©Xk­	è
ýr”Ï0{ ´ �NA–Ï‡ˆûy
0�kAY  œ1p¡Œ�Û
APtÆ… ü ât'‡(ÚVÆWý
¥�\ÄT  Ÿ ù {÷ÝàZ	µ	>ã½òõøûþ8§°¼†Ñþ(¦©õ$� >¬i0�ØH�Š]	eòUÍurA›¡§ ‰�Ô ªêô[!=
·¬ 
 Ž S
Nµ?Êu
Šš	QõžÙ œ J#Ã3	T	·
µ À±m„»qÚ)j„©÷	¡™Ÿ.˜Î 7 ¡‹©!Å¿ÿ	R
ˆ
L»ˆúX·«`¯g EN½ N Š .s!;Uì6/	 
¼
p�è…�VÊER ¾ Ë ÄGâ	Æ	
È
$Ÿª¶Á¦¯à2„Éç�A¶  •Û¤¬X {?Ÿ]	e	£
�ãÑ.¦ Š”TŸÈæ < Ûgy³Á*NÔ�rrk9Ç #—ö°'h
H™”º$5§Br|®Ä™ ÿeÅÆ­ØâtÔ	×
KJ"{ñ£ 55��¶·s©	æ	
Â\ à	E¹_NÌÖÜ  } Ì>Þz§-Z�Ï}) � ¥žþ6À2¿æð3X»Â×ÇW	FºSìïò]ô7¿ÓÖº  E o†á_cn…¾~º.
`
w
1³îRfÝæûèì‚ƒ ˆ ÷ qyào4=�õº
¹´,X¥3Ï&P¤Âï?‹ÔÚ/ ` g n p † So°\„ÊwÞüˆ©·ú^	y	˜
v‡Íw*@IV H]™ÏOmm�è±Ò …©YèKë>l�Ìþ>ûœŸµm	p	{	|¢ÀÃä³ª & i ï $^²·CzŽB	i	m
±
ÏèQ;uêªú ùæ#Fu”Ò6�]C�)3?Ü'e’œé ŒÕ)¯ÑPgAy¤à+iÛ1, , J b ƒ é +ˆ~2twòõ+\o¦¸95:[	P	D
å
x
'YHù!*³áå -ùÌî
N«†  xšýOd³)’Ïüê	éËBÏÛ©îLXdi›  ¸ È^¹ Šš«ËKn­¹.	6	°	$
\
Ç%Êµ"îƒ‘÷ È w&UõCÅ1Ì+
‰ÞœÙ=Kç,;h¿³o‘  Q r ÑìQ	•¢	
š
¿
5·ŒcÆŠJ‡ÑÝáU  å eó“¿>râ`ãô
Çvø_ÿÊ- 1Ué³ÌíùÔPS‘
l½œ·od¾ 4 !ÛñÊ	ÿ	vnOc¸ñS¢œÞ,ƒ Ü Lr%íâtý"Š®ù…	¯í Ë  “Qy%½s
µ
Jcƒ˜RO Óê0Ð<†Õ/I›Å)
ËÜšJµÛ ð;Kw·Ñ,	�
*È˜Ü0vx¬ DäM½wëö}	Ž	QT#â7^þ9éöÂ�’ª­· [ @k”r	‘	Í
Ò
	&|9 ³ @¨Î‡Ú
¤ŽÃXz 	 ž «/×MÜ´	~ñ‰ñÿýô lƒþö	M—®f ÃTÓ	.	Ÿ
Ð-¥‰q®ÈÆ | þµÅsyG˜,ÎD|#zA^# ö ¤¯[ƒbmÉ¾åÁô¹V	¹
²Eú¹ç˜2l£¾Ò¹ çJ�“Ë=\ž´l	o	B£~Âã‘© · *‡Ç*cnŸ²Ê¬45	ÉÊÎ¨h) ò¾Ó R¡	
�
¼ØÅæu÷‰àÉ  sÔ.ø„	É	þ	´
N·ìðÿ¡Ê+ ² C“†+	�	�	S"6ý)8õ�Á%‘¬{uy Â´ÄrÛˆˆLÇüÅ £½æl½Ò«ó3	k	�
¢×ù½ÈÑ( B¢³Ó -’*	ƒ	³
!‡ü7M¡¶Öëøþ ÆûÐ+ AFG|åæç�y+,de(	)		€	�	‚	½	à	=hñò`a†­êûUV�‚ƒÐSïùú ( ¿`zf4
V
%{óbj”®¹Äâëö©”9Ñwˆ¬í "  Ø ñ H`×}¼Þè Ž�D	H	¾	á	ò	
ª
ß
>i-äf›W„ÌT’ð= ) 0 k ‚½O™W
e
Â
Ø&UFKŒ6 Ya{ÜÝ	ù	VÜ€ôckÙ ?{~´î ¹5
í
£|9=•¯º'®:Òãô~x &©SÀKígo
|
ÑžÅãì÷„ªff•Ä‰ß­ü T ´Øf~½d&ø¹	

ßåœ;Gàëûšñ¹ì  Ù �“ºßó9«E	Ú	ó	;
«
?j¯g&Í/>c 	E!-lç¿	â	à
.ÃÉ2˜   Î ò Iaùo†é�%‚F~I	fÕXx°0…”¯U“×" ] ûšö>
N
X
Š
®
Ô
!R¿÷i˜ž¤´u­Lb
² 1 /ŒP–·6`„×	
Ã
Ï
Ùû*B•gv  ¼ ¾ÙwÁf
Æ
ŽVŽ?�¥7L $ * H l ƒçAð›Ã1mB
–
ò
tì'#G©„Þ± G�lÚo�ýj ö�Þ	ð	T
c
Ý+d dô|)ë ” Á 
L
Wõ	Nm@¹cï ¤ ¬ Z[L#b|Ý–Øú	ªJµ  %BU…
m¤Šü–²BG;qäLyž u ºÐïÖ®±t	1
N}Ý:4Ó	… iÂ ß h3	Î	H
î
½õXlPZ}»ÎÄÓÖDl {£
6
E_’—8C>°_(¯õå 9  �òè�¥Lhp’�
¶Ÿä«�,5e~G®  X Õ '>T}€«Ô8ƒÇî_p
ÒÆø`|…¥ôAnÀ•ý`ý – ÝªâD	ˆ	›	}
²ìg–Å»ÔŠà”ñ Ã H–Áåe=	“	4Yºíg.5^‡(ê#; Ì‡ªßë¤	
é
àæÐü| U ¦ © Ž‰'TYC‹º	Õþs•¢º ÙgeÄ	
�<Hsá›ä \µ±]¾0Zùlw	®	%ì*1òkí� µ Ú ‘O»à:°iéˆ¬Ïüô	Ë
@°àQPM?d  ‘bÐbF	Û	ü	z
h'¿Îò-0Ý ”ôB™î	<
¬
kL{‚l	 Ê—‰ñ71BZ  B :�2ÈCq%Gž˜¥ã	á
.ÄÇ3™† .�mèóÜ
¢1p¦	3 +Ž/_9QuŠ 
ÌF"¢ÏÀ	'NBÊö x ® ý b×ãuWNpêid~&ÓXM	>¦Y·DqÅ# ! Ï Ò ó JÔÓÛWR˜J	þ
+¬Öy14c•âD”ØU¦ ˆ�Ú 	g�3]VX Mú(ü)\‡±ƒÇG±9†�Û°\ d   ë b7O
Y
‹
�v¨Ì›öˆ   ú Ÿ€¹-¨¶	?
’À#?DIÿ%Ž$ L Œ <ÊE›<§Þ	1	R	[	Ò	Õ
"æSøóöùüÿ™Ÿ¥µ®‡Ò§ª³—) ^ QuŠü4÷!÷î';|øšá¯
ã
zjä¾Vb9[±½Mc)5Nbßãç 2 ­î#‹-‘aI
w	
Ð
Î+CsBz¨– . ?Qj—¸7I‹^Ù	Ø	¨
fÚS’v¬#Š�Õw  �1^‚;	™	F
Ä
]rêóùVbŒþ!£=oœ¢hJûJ 0'Ù…• W¬	ç
üqÎO[sS
  G\<>q@Ž# ¿º D"Â‡
 ‡W˜Í ½ -«ÚWxëÀd	
¢
Ç
o€œ�©ÀßjæžÉ8µ  4–¶õÝå	g
¦�ß¦¸{­MË D m … � �„2Wn¨º¶]	v
—
0u†í)H
RëëÙ % „B®yÐåœO@h	�(ÛßhÚ0 + I è svñô8Ë	é	#
C
$ª´…XöG   P 0èT=qÄË¾
ó
4¶Æ‹›²TŸ, ÒK/H$Ä(
‚mÛ	 õP]Ç—ŽÛ¶ � ¤p­þ ÆI³4	U	¸
âægˆk¸	 Á¼r…È	5É*Ä ²*‘ß	ñ	3
U
Þ
,eš¬¸Ãõ6 ¨µÿ8ÅË‡Ïì �¼Ûø	d
¢<ƒE}³5 Í n…ò,÷kªæ®ÞeÔ/™ .Ø0À
M
‰
ñ
¾ö
¨¬a° À "�K
S
ón¸übŸ ¢ 	
M¼k‰ûOY3±AÕChk 8 • Â =ñŒªÆädo‘	€
X_4€†ºéd¿ð ¥ [\Þêv	£	r)¡€ ÉÎðí	û	Ê
ßKPO¾ B$F&¡ w ­ ûM[°hc}—ÒÙ	«¶Ú ‹ tí	0	Ñ	å‘"�CHËMŸ í"&vØ:	˜	qéøap‹ý RÍ«<NZnrI VµCc	†
¡
nŸ¥†—¨¿Þå�z O /œ­<V7g	"
½
Ž3µ³WÚêFS ÅÑH²O\Æ–áåf· Ì €»ñ+Ç	2
­~;«·ÂÓ5DÈþ7ÄÊ
†Ã ¿ -<ð× ãc�
J
ð
jò§°3…Ôèž¾Bj v AZ¯ÈgÑ	u	ì	É
%Þ Ù 	b	f	—	Ð	žä÷2oQ§¾!ŽÝ ;YÿfI
ï

¬[ÒÅ•ä6%  à i45@A^	_	`	a	ž	Ï		«ö1mnÒëì/0¼½ÏÐÑ‹Œ�Uãm$  á –jzÜ¥­6¡|B åþŸ	¤
¾YÓí1~zÕVÔ× YÉçEn â k{¤ÂÝ ®}´í+#	“¿±`Ö)Œ¡Z  |öz¢QO
`DÚî?{™€ 87æÿÕ 	¥
9ZÔ2l°ûSkwÁØèãFoÈ = —¦ÜhŽ”C¡Â7
ù
F‚˜ssÑ;WÕðöæÊ: ™ l�¥Þ¡¦~÷­µî$	i
šÊå¬×*-�¢Ï ài‚
”'•$[ 6H¯Å : !HÃ¯Žž±³(qÀI‚»6C  ã $|˜·óéMu˜¤,“^
·Å ²af…%[¨s}�æš¯ø% Y ( Ç�ÕÙã9„ÈïuP²	q
Ø
aÉEÇa|·Ìïš¦õH�Áò  Ö ÷»$Ñp‹	
ïùK†‹yù|–aþ º }Î?f{~£¬Õ	PÛ@Ì“þ 8ÊÆU`R®œÍ`	Â	)Ó<L]}#ðBo¤ô `‚·ã:E“¸			ª	ç	¦
ho¡!T`xé`Q•É×Ý ¡	&
~
3]±Ù' 69«žŒtÖ8	œ	 
÷
³—Æáü
lÂ¼áG  — 6hÞ8šœ»ç ‰	Ã:[ÕímF¼Õº‹äOpÍò  ~ 	§Ý�MÃßf>	ú
th/~ë; IiæD0{8
G.D6<‹‘Ðñ)[ —•½”	Zƒ™¨»×twÒXˆÖ¾$×ç > Ä j˜Í?Â¢¢¯é5€î[_÷<Ër € ¦Ù7rÇçñøï¥	
j
9ÑØ€ŽÁõ8» ‘ ¦ÁßÍ3¥ÀÕ%	ê
@›G­jé+..?r  š Ÿ¢§ˆ«Y®ÃØàh‚ÈX	‹áË »Tó^µ×} Wm‘ß÷ÿ+4¶¼ì@	çæÛ=F£íðý€ÀÔ=Ð z pâ`(¿Z»ïÿt––ç7 ° dD»	Ö„àSÞü%éIí»Æ F V ª yåŠY/
a
ƒ
”
2•´¨(gÂ\!s£ƒ“°	  § �›á‡o†Uj†ÅVŒx
ü
€j„ ø h©�´ö=tâ7¦»Ð'¥Œ I°Šp(ºž-¼ ; ‰ Ê rÚ fŸz²5>ž)MÅ	»
žµ“1ƒœÇåDQ@ 4"Ä€×ár
ÁJI[Y¹4qÃðÕÛ# o ‡ np…^¿1vxß3x	-
_
¸v³+?W\Îó%l~Žçî‚°Ó h ]±¶(êEkýf€úz	Î¡x2&P©té›ù( a ÿ ]w}™¸üN[‰‘™¥ªm¸Îû-Z	O	¯	wÆ	&AM!ííš
² q Ç ä Td%²ÐôËBPìOá_”™
ˆ<Jb+I^g†Ðnn�! Û ’Péìá;Ú…ðü!‰š­r
IbÊ®bRQ›IN´‚Ú Z L¼?Í:?jêv¶Ðq	|	œ
Ì
Ù
£á«ÂW ªýSk‚Ö
ý	³	õ	}Fð–@]óe! ¶ õ )†ZÈmž±äÿÉQ� n	A±È}¸ÁÄÍå§öß¢¨ ¼Ñq2j	Œ	ý	(‡Œ À  ' × ð _Ö{ÝÒcC	G	²
ðiéúL€“ÏR<v«ÿ j %X¸Ü	n
{
Á
ÐØe>ezó}Þû  S ³øD�’c%}
:
FÈú!.1—ëb  # » @ú•v¶õšÖ	=
­
 QA�ƒ—³”Ýfu
 KFï	
*MŒ(ê t ~ÏgîÕ$­TDl‘7Ü|Í^Òäÿ�Á Ô çG|•¤ÓC7‚^ ¹{ó+4@mÓF_“ �°ËSY
BŠÃ	Ô$ãj a˜¯hèaz6ñL,Ü A 9Ë*Ç�Î—¤ò-M^0o¥8‰2õ Ñ LÖÓVÒQL	*=Ž~38b2CpáCW[¥,  K c ê ;P‰žaÉ3D¸ö ;,&:§6™¦Q	â
yUaZ4aš‡–Þâæ - Š,]”HŸV	«	E
§
æ
\‘¢"yIúR ,3•ÿF¹¿Ü;ä	›ipÞÈÌ"´Ê C „ ç ruxƒÏäóp§¹Ê\
		è	/…Å(êg+/Ø .ô'
£¬‡Ú	 åÝ
4‚§´²4 /Žn
½2^ûa ú
Íéï¢	qO(
 ì›¬´u×9	!
`´ÌªMYmœÙéH ,@Çð*Ðb�ë	iñCÇØýeÃ½ :yþeäý–	�	ª0êyÔÜâ5X õ7ÁŸP�“ì"	ø
ÙÐ:˜ïúRjvÀ‹â ˜ ßG¶�t—£¬²]
ÄÉ&#Z„ŒåÎ÷# ¹ 7ÉÍº_#›ÌÐoŠ	±	Á	(È;O\ËŠ¶ËîxGï’øó g_9™›’‹º	7	%
ö
n _&»P i¡L®¼è/ÖCvŠ½ÖZq   Ø
Vö*Þq¤ÆÔg�?	8Š?Úiè-<EÀ>q´< y ¯ xšäX…ÄU“
û
§ƒßÁir’ É 3¨‰Ö'LZ�’0¸ºÆp  Æ þ cm'vÏADOëûje�2NÍàYN	,
u;>LìÍ$	 ô üRj
	 ™	›
H­•ÙÞ\¡V§ R WÕÜ1|9
À
×EdŸÇ=d’   " s Ó íÔJµSE
ÿ
6¸z]ŒÑò*‹–²ÒãE^tÀ @ Ð KÒXç–£K	,�/n257aKˆâV æ O”‰Îþ+U¦¾ÉÛ[„š�ÝfË.	 	ä¼�³ú` ëôÀ?Æïãëa!	•	©hØðxÓ×ù4¿ØèW fµ^˜s‘–±õ
Èu‰Yw‰^% Y Å 2l™Î)@ˆú£Õ„KT€’
7‚À:‘¹=pÌ ? Vû´m\ž`±]s¿ Nê´Íý*]ÅîT¥°Èêï€²ˆÜùe k²³ÃÄQRS£¤HIJ6�¾¿‡�ø¸  +Z  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                       '                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 (                                                                                                                                                                         	                                 	            )      
                                                   
      	                     
         
                     #       )         /                  Y   @               
      ?                                                                                                                                                                  '                                                                                                                                                                                                                                                                                                                                                                                                             (                                                             	           	    )  
                 
  	       
   
       #   )   /      Y @     
  ?   f  g  g8  g^  j¢  nÈ  sÎÏkAt¸Eoy mjÅï£'Íä[§¸L’¹ºèEÛëm
: J8· lîIWõááb<Ïª[` g i ÷ U©Uoìõ-n…�Â4p	‘
±
‡Ýû@BºÆÛ?J_ªdÊ!ª±– ¡y
-„y
—-y
---# A ÒYT—ÑOO ¸	:2/@ R =VÝ³
ÜÝ8	6Cµd‰ÇŠ¢%šµW *¡�	$z¢<%ò!ÝhRÔR™G\Î�ìÀDØdœšu9½4
ì
®¹&­ 	"˜RG\ÎÁ[˜Çí¢N?õÙN?Ù7¼º}:	†N?Ù¼,Z
»��¼�
²RG\Î± x5�Ü¢ ÖH£û™†ûÍOû_ R[É‡ùG\˜ÎlRÔRË &=#Jq™®º›,G\¶Î�¼ ZìuºoŠ	Š´rN¥67ÈŸ–²ºÀC� 	ÝÓf>hi TUõ‰Ù;D³n b é 5Ïg/á®V´4Á§–²^
N?Z
»��õÙ¼Õ	@“!Ÿ–²‹ö
tn å  ˆ¥Uš¯èõ2_õù¾9i	\
x
Ù†;Yƒ³ÜÝ7i -ºáå–²ºË u™‚ÜÛà†	‘	°ê,&9E fç©|A -76Mß“  	"˜Z�n	Áç ruó	&­'Ë Ë ù X!N?™->uÔÙ˜Þ·ÝŽRîtÖ8	‹	Õ	^
ZÓ@h‹lÙ“6GíÃ{8
ú
D~�™UõÙ;)†mäYN	¸	Î)†mìºouŠ	ŠÇ?[ â¥[­›DMÍ Ô/ˆE÷"ÙÛà†	°ê,<	’	3-]'":O ™¦H­Ù5ÂE¹NÌ§ƒ …Ytwòlo�þ¦¸[œŸm	'ÀÃä*û sË &=øk›¶c¼½ Ø)q£Æ€78À=p=€‰dTÌš ¦ˆÓU)<}Bo¤û sË&=…Yl�þœŸm	ÀÃäƒ twòo¦¸['*Ó ÿ
¸zò*ÒE^\ Ó
h�£t	±ëæüÈ œoƒ …&YþtwòClo�þ3¦¸»1Ì[œŸm	+
'=KÀÃäìï¿Ó* OØ)q£Æ ™¦€78H­À=pÙæF”Ò6�]3?’G æïÂlA
•
së" :�ÈC%˜¥á
3™†üRj		•\î4Á@
G•¹±	È¶ËîGŠ,HyÄÌ>Z2‡ªß&üã	ô	àY·ÇÅ?d|Š,H±	È4¶ËîGeyªÖ³	}ðóA 9Ç�—¤-/k­	0àX3ÂïÔÚÅ lÎ@ú:Ì¥6æðô7º{ z¨RJ¡ìx^–B—�+AÃÍC{"y§ "‡j¹4qÃY ¥“9uÃ$	ª	¦
Ø
ú
š¬*¡!-x~ÁQôÀë!	Øù¿÷ïÅm„Ø­j©	È
$KŸ".˜£± �	Rô$�«ztx6žŒ÷
—á{ x—+ÍC{"y¡AÅØ­K"£z¨RJì^–B�Ã&þC3»1Ì+
=Kìï¿ÓA 9ªÇÖ�—¤³	-}ðóP–·6×	Ùv|z¢Ú?Ìé
§/o ‡ np…^¿1vxß3x	-
_
¸v³+?W\Îó%l~Žçî‚°ÓÆ þ cm'vÏADOëûje�2NÍàYN	,
u;>LìÍ$	� ¥žþ6À2¿æð3X»Â×ÇW	FºSìïò]ô7¿ÓÖºEN½· *‡Ç*cnŸ²Ê¬45	ÉÊÎ¨h)` g n p † So°\„ÊwÞüˆ©·ú^	y	˜
v‡Íw*@IV H]™ÏOmm�è±Ò.ô'
£¬‡ÚW ÜŽá<	‡	’	š	3µ±ë-]¤'"}:”üv’ë ø0â*
Ýk›:¶¾þÜc½  Ø
Vö*Þq¤ÆÔg�?	8Š?Úiè-<EÀ>q´< # » @ú•v¶õšÖ	=
­
 QA�ƒ—³”Ýfu‚ · *v‡Çò’¾ë ,1øø*cnŸ²Ó (·Ê0J¬49R¡âí	5	
*
[
�
ä
	èÉÝk¼ÊØ›ÅÎÚæu¨÷KWch‰:‚�¶¾þÜàcÉø)½àä # » À .@ú•ØKv¶õš"0F�ÀÖ	ï	

=
K
M
S
‰
­
ñ
 *Q¾ö
AMón¨�ƒŒ—³¸üb”¬Ý(afŸ°uêÙgeÄ	
�<Hsá›ä% „B®yÐåœO@h	�(ÛßhÚ0¹5
í
£|9=•¯º'®:Òãô~xfµ^˜s‘–±õ
Èu‰Yw‰^% Y3 q$Ïx¼u—™ÝÌ €»ñ+Ç	2
­~;«·ÂÓ5DÈþ7ÄÊ
†Ã· *‡“ÇQÙgˆe*cn�Ÿ²Êy¬Ú%4½ 	5	Ä	
s
µ
Jcgƒ��É<Hs˜ÊáÎR¨3]hOV›äX)ài‚
”'•$[ 6H¯Å   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #·<?xml version="1.0"?><Features nextValidID="15"><Feature recentID="0" name="lacI" directionality="1" translateFirstCodonAsMet="1" translationMW="38559.52" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5008-432" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli. &lt;/i&gt;This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"/></Q></Feature><Feature recentID="1" name="KanR" directionality="1" translationMW="30979.95" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2569-3384" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (GeneticinÂ®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="2" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1859-2447" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3477-3932" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="SUMO" directionality="2" translationMW="11204.88" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4148" maxFusedRunOn="4148"><Segment range="4196-4486" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;S. cerevisiae SMT3&lt;/i&gt; (truncated)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;cleavable ubiquitin-like protein tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG"/></Q></Feature><Feature recentID="5" name="rop" directionality="1" translateFirstCodonAsMet="1" translationMW="7196.20" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1241-1432" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;rop&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Rop protein, which maintains plasmids at low copy number&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL*"/></Q></Feature><Feature recentID="6" name="bom" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1534-1673" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;basis of mobility region from pBR322&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lacI promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4930-5007" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T7 terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3969-4016" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;transcription terminator for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4578-4602" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="RBS" type="RBS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4541-4563" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;efficient ribosome binding site from bacteriophage T7 gene 10 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/2676996&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/2676996&quot;&gt;Olins and Rangwala, 1989&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="TEV site" directionality="2" translationMW="899.99" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4168" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4148" maxFusedRunOn="4148" detectionMode="exactProteinMatch"><Segment range="4166-4186" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;tobacco etch virus (TEV) protease recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="ENLYFQS"/></Q></Feature><Feature recentID="12" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4603-4621" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="6xHis" directionality="2" translationMW="840.86" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4080" maxFusedRunOn="4080" detectionMode="exactProteinMatch"><Segment range="4083-4100" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;6xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="HHHHHH"/></Q></Feature><Feature recentID="14" name="6xHis" directionality="2" translationMW="840.86" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4148" maxFusedRunOn="4148" detectionMode="exactProteinMatch"><Segment range="4505-4522" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;6xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="HHHHHH"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ·<Notes>
<UUID>62d00644-8f27-40ef-9b15-3f382df4e87e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pET His6 Sumo TEV LIC cloning vector (1S)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="10:32:59">2018.9.17</Created>
<LastModified UTC="11:59:36">2025.8.26</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      HO  d               E              ÿÿÿÿYô? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
